Can I read FISH for Pre-implantation Genetic Diagnosis on EtoBox?

FISH for Pre-implantation Genetic Diagnosis by Paul N. Scriven; Caroline Mackie Ogilvie is a book available to read on EtoBox.

What is FISH for Pre-implantation Genetic Diagnosis about?

Pre-implantation genetic diagnosis (PGD) is an established alternative to pre-natal diagnosis, and involves selecting pre-implantation embryos from a cohort generated by assisted reproduction technology (ART). This selection may be required because of familial monogenic disease (e.g. cystic fibrosis), or because one partner carries a chromosome rearrangement (e.g. a two-way reciprocal translocation). PGD is available for couples who have had previous affected children, and/or in the case of chromosome rearrangements, recurrent miscarriages, or infertility. Oocytes aspirated following ovarian stimulation are fertilized by in vitro immersion in semen (IVF) or by intracytoplasmic injection of individual spermatocytes (ICSI). Pre-implantation cleavage-stage embryos are biopsied, usually by the removal of a single cell on day 3 post-fertilization, and the biopsied cell is tested to establish the genetic status of the embryo.Fluorescence in situ hybridization (FISH) on the fixed nuclei of biopsied cells with target-specific DNA probes is the technique of choice to detect chromosome imbalance associated with chromosome rearrangements, and to select female embryos in families with X-linked

Author

Paul N. Scriven; Caroline Mackie Ogilvie

Publisher

Humana Press : Imprint : Humana

Published

2010

Language

EN

ISBN

9781607617891

Subjects

Science, Chemistry, Medical