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Localization ofo-glycosylation sites of MUC1 tandem repeats by QTOF ESI mass spectrometry by Franz-Georg Hanisch; Brian N. Green; Robert Bateman; Jasna Peter-Katalinic is a Chemistry article available to read on EtoBox.

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The potential of electrospray mass spectrometry (ESMS) for the sequencing of glycopeptides was evaluated using quadrupole time-of-Ñight (QTOF) technology in the MS/MS mode. The location of O-glycosylation sites was possible in the positive ion (+ ) mode by detection of prominent y-and b-fragment ions from the underivatized TAP25-2 [ T1APPAHGVT9S10APDT14RPAPGS20T21APPA ] , an overlapping sequence of MUC1 tandem repeats which had been glycosylated in vitro by two GalNAc residues in the positions T9 and T21. The high mass resolution and accuracy of QTOF-(+ )ESMS allowed reliable structural assignments. The reduced complexity of the fragment spectra and the higher signal-to-noise ratio render QTOF-(+ )ESMS an alternative mass spectrometric approach to the identiÐcation of O-glycosylation sites when compared with sequencing by post-source decay matrix-assisted laser desorption/ionization MS. Diagnostic ions from the N-terminus in the b-series o †ered direct evidence, which was supported by indirect evidence from the C-terminus ions of the y-series. The higher glycosylated fragments were mainly observed as multiply ionized species. 1998 John Wiley & Sons, GalNAc 2 -substituted ( Ltd.

Who reads Localization ofo-glycosylation sites of MUC1 tandem repeats by QTOF ESI mass spectrometry?

Author

Franz-Georg Hanisch; Brian N. Green; Robert Bateman; Jasna Peter-Katalinic

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley-Liss Inc; John Wiley & Sons Inc.; Wiley; Heyden And Son (ISSN 1076-5174)

Published

1998

Language

EN

Field

Chemistry (Physical Sciences)