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Ultrasensitive fluorogenic chemosensor based on ESIPT phenomenon for selective determination of Cu2+ ion in aqueous system ... 1 1 Introduction 1 2 Experimental section 4 2.1 Materials and instruments 4 2.2 Synthetic procedures 4 2.2.1 Synthesis of 2-(2-(4-nitrobenzylidene)hydrazinyl) pyridine (1) 4 2.2.2 Synthesis of 4-((2-(pyridin-2-yl)hydrazineylidene)methyl)aniline (2) 4 2.2.3 Synthesis of 5-(diethylamino)-2-(((4-((2-(pyridin-2-yl)hydrazineylidene)methyl)phenyl) imino)methyl)phenol (PS) 4 2.3 Crystallographic measurements 4 2.4 General details for UV–Vis and fluorescence measurements 5 2.4.1 Preparation of the stock solutions 5 2.4.2 UV–Vis absorption measurement 5 2.4.3 Fluorescence measurement 5 2.5 Fluorescence quantum yields calculations 6 2.6 Theoretical calculations 6 2.7 X-ray absorption spectroscopy (XAS) 7 2.8 Cell viability and imaging experiments 7 3 Results and discussion 7 3.1 Synthesis and crystal structure of PS 7 3.2 Photophysical properties of PS 8 3.3 Sensitivity of sensor PS toward Cu2+ ion 9 3.4 Binding studies 9 3.5 Theoretical calculations 10 3.6 X-ray absorption spectroscopy (XAS) 10 3.7 Selectivity of sensor PS toward Cu2+ ion 10 3.8 Application of Cu2+

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EN

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nonfiction