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Concomitant Changes in Mitochondria and Intermediate Filaments During Heat Shock and Recovery of Chicken Embryo Fibroblasts by Nancy C. Collier; Michael P. Sheetz; Milton J. Schlesinger is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Concomitant Changes in Mitochondria and Intermediate Filaments During Heat Shock and Recovery of Chicken Embryo Fibroblasts about?

## Abstract Utilizing video‐enhanced differential interference contrast microscopy of chicken embryo fibroblasts, we observed dramatic changes in the localization and morphology of mitochondria shortly after cells were subjected to a __mild__ heat shock. At normal temperatures mitochondria were distributed in the cell cytoplasm as elongated, tubular, and dynamic organelles but upon heat shock they moved to the perinuclear region and formed a tight ring of short swollen and—in some cases—fused vesicles. Vital dye staining of mitochondria with rhodamine 123 and indirect immunofluorescence staining with antibodies against the mitochondrial‐matrix protein, HSP 60, confirmed these results. Using cells double labeled with antibodies to vimentin and the HSP 60 protein, we found that the changes in mitochondria were accompanied by perturbations of the intermediate filament network that we and others have reported previously for heat shocked cells. Microtubules remained largely unaltered by our heat shock treatment and the redistribution of intermediate filaments and mitochondria occurred even in the presence of taxol, a microtubule stabilizing drug. The effects of heat shock on mitochondri

Who reads Concomitant Changes in Mitochondria and Intermediate Filaments During Heat Shock and Recovery of Chicken Embryo Fibroblasts?

Author

Nancy C. Collier; Michael P. Sheetz; Milton J. Schlesinger

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

1993

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)