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Dual-Modified Liposome for Targeted and Enhanced Gene Delivery into Mice Brain by Bruna dos Santos Rodrigues; Sushant Lakkadwala; Takahisa Kanekiyo; Jagdish Singh is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Dual-Modified Liposome for Targeted and Enhanced Gene Delivery into Mice Brain about?

The development of neuropharmaceutical gene delivery systems requires strategies to obtain efficient and effective brain targeting as well as blood-brain barrier (BBB) permeability. A brain-targeted gene delivery system based on a transferrin (Tf) and cell-penetrating peptide (CPP) dual-functionalized liposome, CPP-Tf-liposome, was designed and investigated for crossing BBB and permeating into the brain. We selected three sequences of CPPs [melittin, Kaposi fibroblast growth factor (kFGF), and penetration accelerating sequence-R8] and compared their ability to internalize into the cells and, subsequently, improve the transfection efficiency. Study of intracellular uptake indicated that liposomal penetration into bEnd.3 cells, primary astrocytes, and primary neurons occurred through multiple endocytosis pathways and surface modification with Tf and CPP enhanced the transfection efficiency of the nanoparticles. A coculture in vitro BBB model reproducing the in vivo anatomophysiological complexity of the biologic barrier was developed to characterize the penetrating properties of these designed liposomes. The dual-functionalized liposomes effectively crossed the in vitro barrier model

Who reads Dual-Modified Liposome for Targeted and Enhanced Gene Delivery into Mice Brain?

Author

Bruna dos Santos Rodrigues; Sushant Lakkadwala; Takahisa Kanekiyo; Jagdish Singh

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)