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What is Revolutions in Rapid Amplification of cDNA Ends Ne about?

The document reviews the rapid amplification of cDNA ends (RACE), a PCR-based technique for cloning full-length cDNA ends after partial sequences are obtained. It discusses the common issue of truncated cDNA amplification and evaluates various modifications to improve the effectiveness of RACE. A detailed protocol based on RNA ligase-mediated RACE and reverse ligation-mediated PCR is also provided, along with a critical analysis of existing methods and their challenges.

Author

mrympaknzhad777

Language

EN