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Structural analysis of gellans produced by Sphingomonas elodea strains by electrospray tandem mass spectrometry by Virgínia M.F. Gonçalves; Ana Reis; M. Rosário M. Domingues; José A. Lopes-da-Silva; Arsénio M. Fialho; Leonilde M. Moreira; Isabel Sá-Correia; Manuel A. Coimbra is a Materials Science article available to read on EtoBox.
What is Structural analysis of gellans produced by Sphingomonas elodea strains by electrospray tandem mass spectrometry about?
A commercial gellan sample (Gelrite) and a gellan-like polymer (JB3) obtained by exposure of the producing strain to chemical mutagenesis were subjected to partial acid hydrolysis and the resultant oligosaccharides were identified by Electrospray Mass Spectrometry (ESI-MS) and Tandem Mass Spectrometry (ESI-MS/MS and MS n ). In both gellans, the main fragments were in accordance with the tetrasaccharide repeating unit, D-Glucose (Glc)-D-Glucuronic acid (GlcA)-D-Glucose (Glc)-L-Rhamose (Rha), described for the wild-type gellan, showing the higher acid lability of the Rha-(1 ? 3)-Glc linkage when compared to the (1 ? 4) of the other residues. Under the experimental conditions used in the study, as expected, no acyl substituents were observed in the commercial gellan but in JB3 oligosaccharides a glyceryl moiety was identified, substituted in the 3-linked Glc residue. Furthermore, the analysis of the MS/MS and MS n spectra of both gellans allowed the identification of structural details, some of them not yet reported for these exopolysaccharides. The presence of oligosaccharides with single Glc and Rha residues substituent of the tetrasaccharide unit of gellan may represent novel side
Who reads Structural analysis of gellans produced by Sphingomonas elodea strains by electrospray tandem mass spectrometry?
It is typically read by researchers, students, and practitioners in Materials Science.
- Author
- Virgínia M.F. Gonçalves; Ana Reis; M. Rosário M. Domingues; José A. Lopes-da-Silva; Arsénio M. Fialho; Leonilde M. Moreira; Isabel Sá-Correia; Manuel A. Coimbra
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0144-8617)
- Published
- 2009
- Language
- EN
- Field
- Materials Science (Physical Sciences)