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Integrin Trafficking Regulates Adhesion to Fibronectin During Differentiation of Mouse Peri-implantation Blastocysts by Jeffery F. Schultz; Linda Mayernik; Ujjwal K. Rout; D. Randall Armant is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Integrin Trafficking Regulates Adhesion to Fibronectin During Differentiation of Mouse Peri-implantation Blastocysts about?

Trophoblast cells of the peri-implantation blastocyst differentiate from a polarized epithelium, the trophectoderm, into invasive cells having an apical surface occupied by integrins that mediate adhesion to the extracellular matrix. Blastocyst differentiation was assessed during serum-free culture using a fibronectin binding assay with intact mouse blastocysts. Fibronectin binding activity became elevated during a 24-h "window" after approximately 72 h of culture. Blastocyst differentiation was unaffected by transcriptional inhibition with alpha-amanitin, however, exposure of cavitating morulae to the drug significantly delayed the onset of maximal fibronectin-binding activity. Inhibition of de novo protein synthesis with cycloheximide delayed development only when added during the first 24 h of blastocyst culture, indicating that proteins required for adhesion to fibronectin were synthesized at least 24 h before blastocyst differentiation was completed. Since blastocyst differentiation did not appear to be regulated temporally by gene expression, the possible role of protein trafficking was investigated using the inhibitor, brefeldin A. Brefeldin A caused a reversible, dose-depen

Who reads Integrin Trafficking Regulates Adhesion to Fibronectin During Differentiation of Mouse Peri-implantation Blastocysts?

Author

Jeffery F. Schultz; Linda Mayernik; Ujjwal K. Rout; D. Randall Armant

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0192-253X)

Published

1996

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)