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Identification and Characterization of a Novel Major Facilitator Superfamily (MFS) Efflux Pump SA09310 Mediating Tetracycline Resistance in Staphylococcus aureus by Daiyu Li; Yan Ge; Ning Wang; Yun Shi; Gang Guo; Quanming Zou; Qiang Liu is a scholarly article available to read on EtoBox.

What is Identification and Characterization of a Novel Major Facilitator Superfamily (MFS) Efflux Pump SA09310 Mediating Tetracycline Resistance in Staphylococcus aureus about?

## Abstract Drug efflux systems have recently been recognized as an important mechanism of multidrug resistance in bacteria. Here, we described the identification and characterization of a novel chromosomally encoded multidrug efflux pump (SA09310) in__Staphylococcus aureus__. SA09310 is a 43-kDa protein with 12 transmembrane helices. The conserved amino acid sequence motifs of the major facilitator superfamily (MFS) were identified in the protein SA09310, which indicated SA09310 belonged to MFS transporters. Expression of the__sa09310__gene was induced by different types of antibiotics, including aminoglycoside, tetracycline, macrolides, and chloramphenicol. The__sa09310__gene knockout mutant (Δ__sa09310__) was constructed, and its susceptibility to 30 different antibiotics was evaluated. Mutant__△sa09310__exhibited increased sensitivity to tetracycline and doxycycline, with 64-fold and 8-fold decreased MICs, respectively. The mechanism of SA09310 mediating tetracycline resistance was demonstrated by its ability to extrude intracellular tetracycline from within the cells into the environment. The efflux activity of SA09310 was further confirmed by EtBr accumulation and efflux assa

Author

Daiyu Li; Yan Ge; Ning Wang; Yun Shi; Gang Guo; Quanming Zou; Qiang Liu

Publisher

Cold Spring Harbor Laboratory

Published

2023

Language

EN