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Hemin-incorporated Nanoflowers as Enzyme Mimics for Colorimetric Detection of Foodborne Pathogenic Bacteria by Wang, Kui-Yu; Bu, Sheng-Jun; Ju, Chuan-Jing; Li, Chang-Tian; Li, Zhong-Yi; Han, Ye; Ma, Cheng-You; Wang, Cheng-Yu; Hao, Zhuo; Liu, Wen-Sen; Wan, Jia-Yu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Hemin-incorporated Nanoflowers as Enzyme Mimics for Colorimetric Detection of Foodborne Pathogenic Bacteria about?

Rapid, sensitive and point-of-care detection of foodborne pathogenic bacteria is essential for food safety. In this study, we found that hemin-concanavalin A hybrid nanoflowers (HCH nanoflowers), as solid mimic peroxidase, could catalyze oxidation of 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS) in the presence of HO to a green-colored product. HCH nanoflowers, integrating the essential functions of both biological recognition and signal amplification, meet the requirements of signal labels for colorimetric immunoassay of bacteria. In view of the excellent peroxidase mimetic catalytic activity of HCH nanoflowers, a colorimetric biosensing platform was newly constructed and applied for sensitive detection of foodborne Escherichia coli O157:H7 (E. coli O157:H7). The corresponding detection limits was as low as 4.1 CFU/mL with wide linear ranges (10-10 CFU/mL).

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Author

Wang, Kui-Yu; Bu, Sheng-Jun; Ju, Chuan-Jing; Li, Chang-Tian; Li, Zhong-Yi; Han, Ye; Ma, Cheng-You; Wang, Cheng-Yu; Hao, Zhuo; Liu, Wen-Sen; Wan, Jia-Yu

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0960-894X)

Published

2018

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)