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The orphan receptor GPRC5B activates pro-inflammatory signaling in the vascular wall via Fyn and NFκB by Greta Verena Freundt; Friedrich Alexander von Samson-Himmelstjerna; Jan-Thorge Nitz; Mark Luedde; Johannes Waltenberger; Thomas Wieland; Norbert Frey; Michael Preusch; Hans-Jörg Hippe is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is The orphan receptor GPRC5B activates pro-inflammatory signaling in the vascular wall via Fyn and NFκB about?
Background and aims: Atherosclerosis is driven by an inflammatory process of the vascular wall. The novel orphan G-protein coupled receptor 5B of family C (GPRC5B) is involved in drosophila sugar and lipid metabolism as well as mice adipose tissue inflammation. Here, we investigated the role of GPRC5B in the pro-atherogenic mechanisms of hyperglycemia and vascular inflammation. Methods: Immortalized and primary endothelial cells (ECs) and vascular smooth muscle cells (VSMCs) were used for stimulation with high glucose or different cytokines. Adenoviral-or plasmid-driven GPRC5B overexpression and siRNA-mediated knockdown were performed in these cells to analyze functional and mechanistic pathways of GPRC5B. Results: In ECs and VSMCs, stimulation with high glucose, TNFa or LPS induced a significant upregulation of endogenous GPRC5B mRNA and protein levels. GPRC5B overexpression and knockdown increased and attenuated, respectively, the expression of the pro-inflammatory cytokines TNFa, IL-1b, IL-6 as well as the pro-atherogenic vascular adhesion molecules ICAM-1 and VCAM-1. Furthermore, the expression and activity of the metalloproteinase MMP-9, a component of atherosclerotic plaque s
Who reads The orphan receptor GPRC5B activates pro-inflammatory signaling in the vascular wall via Fyn and NFκB?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Greta Verena Freundt; Friedrich Alexander von Samson-Himmelstjerna; Jan-Thorge Nitz; Mark Luedde; Johannes Waltenberger; Thomas Wieland; Norbert Frey; Michael Preusch; Hans-Jörg Hippe
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)