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This document presents a highly sensitive biosensor for detecting L-glutamate using glutamate oxidase immobilized on a platinum nanoparticle decorated multiwalled carbon nanotube and polypyrrole composite. The biosensor demonstrated a sensitivity of 723.08 µA cm−2 mM−1, a detection limit of 0.88 µM, and a response time of 3 seconds, with effective performance in human serum samples. Characterization techniques such as cyclic voltammetry and impedance spectroscopy confirmed the successful fabrication and fun

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