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Can I read Inter-organelle cross-talk supports acetyl-coenzyme A homeostasis and lipogenesis under metabolic stress on EtoBox?
Inter-organelle cross-talk supports acetyl-coenzyme A homeostasis and lipogenesis under metabolic stress by Ramya S. Kuna; Avi Kumar; Karl A. Wessendorf-Rodriguez; Hector Galvez; Courtney R. Green; Grace H. McGregor; Thekla Cordes; Reuben J. Shaw; Robert U. Svensson; Christian M. Metallo is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Inter-organelle cross-talk supports acetyl-coenzyme A homeostasis and lipogenesis under metabolic stress about?
Proliferating cells rely on acetyl-CoA to support membrane biogenesis and acetylation. Several organelle-specific pathways are available for provision of acetyl-CoA as nutrient availability fluctuates, so understanding how cells maintain acetyl-CoA homeostasis under such stresses is critically important. To this end, we applied ^13^C isotope tracing cell lines deficient in these mitochondrial [ATP-citrate lyase (ACLY)]–, cytosolic [acetyl-CoA synthetase (ACSS2)]–, and peroxisomal [peroxisomal biogenesis factor 5 (PEX5)]–dependent pathways. ACLY knockout in multiple cell lines reduced fatty acid synthesis and increased reliance on extracellular lipids or acetate. Knockout of both ACLY and ACSS2 (DKO) severely stunted but did not entirely block proliferation, suggesting that alternate pathways can support acetyl-CoA homeostasis. Metabolic tracing and PEX5 knockout studies link peroxisomal oxidation of exogenous lipids as a major source of acetyl-CoA for lipogenesis and histone acetylation in cells lacking ACLY, highlighting a role for inter-organelle cross-talk in supporting cell survival in response to nutrient fluctuations.
Who reads Inter-organelle cross-talk supports acetyl-coenzyme A homeostasis and lipogenesis under metabolic stress?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Ramya S. Kuna; Avi Kumar; Karl A. Wessendorf-Rodriguez; Hector Galvez; Courtney R. Green; Grace H. McGregor; Thekla Cordes; Reuben J. Shaw; Robert U. Svensson; Christian M. Metallo
- Publisher
- American Association for the Advancement of Science (AAAS)
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Physical Sciences)