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Innovative High-performance Liquid Chromatography Method Development for the Screening of 19 Antimalarial Drugs Based on a Generic Approach, Using Design of Experiments, Independent Component Analysis and Design Space by B. Debrus; P. Lebrun; J. Mbinze Kindenge; F. Lecomte; A. Ceccato; G. Caliaro; J. Mavar Tayey Mbay; B. Boulanger; R.D. Marini; E. Rozet; Ph. Hubert is a Chemistry article available to read on EtoBox.

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An innovative methodology based on design of experiments (DoE), independent component analysis (ICA) and design space (DS) was developed in previous works and was tested out with a mixture of 19 antimalarial drugs. This global LC method development methodology (i.e. DoE-ICA-DS) was used to optimize the separation of 19 antimalarial drugs to obtain a screening method. DoE-ICA-DS methodology is fully compliant with the current trend of quality by design. DoE was used to define the set of experiments to model the retention times at the beginning, the apex and the end of each peak. Furthermore, ICA was used to numerically separate coeluting peaks and estimate their unbiased retention times. Gradient time, temperature and pH were selected as the factors of a full factorial design. These retention times were modelled by stepwise multiple linear regressions. A recently introduced critical quality attribute, namely the separation criterion (S), was also used to assess the quality of separations rather than using the resolution. Furthermore, the resulting mathematical models were also studied from a chromatographic point of view to understand and investigate the chromatographic behaviour of

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Author

B. Debrus; P. Lebrun; J. Mbinze Kindenge; F. Lecomte; A. Ceccato; G. Caliaro; J. Mavar Tayey Mbay; B. Boulanger; R.D. Marini; E. Rozet; Ph. Hubert

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2011

Language

EN

Field

Chemistry (Physical Sciences)