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Development and Optimization of a Reversed-phase High-performance Liquid Chromatographic Method for the Determination of Acetaminophen and Its Major Metabolites in Rabbit Plasma and Urine After a Toxic Dose by M.V Vertzoni; H.A Archontaki; P Galanopoulou is a Chemistry article available to read on EtoBox.
What is Development and Optimization of a Reversed-phase High-performance Liquid Chromatographic Method for the Determination of Acetaminophen and Its Major Metabolites in Rabbit Plasma and Urine After a Toxic Dose about?
A reversed-phase high-performance liquid chromatographic method with detection at 242 nm was developed, optimized and validated for the determination of acetaminophen (A) and its major metabolites glucuronide (AG) and sulfate (AS) conjugates in rabbit plasma and urine after a toxic dose. m -Aminophenol was used as internal standard (IS). A Hypersil BDS RP-C 18 column (250)/4.6 mm), 5 mm particle size, was equilibrated with a mobile phase composed of aqueous buffer solution of KH 2 PO 4 0.05 M containing 1% CH 3 COOH (pH 6.5) and methanol (95:5, v/v). Its flow rate was 1.5 ml/min. Calibration curves of A, AG and AS were linear in the concentration ranges of 0.5 Á/250, 1 Á/200, 0.5 Á/100 mg/ml in plasma and 1 Á/200, 0.5 Á/150, 0.5 Á/100 mg/ml in urine matrix, respectively. Limits of detection and quantitation were calculated in all cases and extensive recovery studies were also performed. Intra-day relative standard deviation (R.S.D.) for A, AG and AS in plasma was less than 5, 4, 2% and in urine less than 4, 7, 4%, respectively, while the corresponding inter-day values were 7, 6, 4% and 5, 8, 6%, respectively.
Who reads Development and Optimization of a Reversed-phase High-performance Liquid Chromatographic Method for the Determination of Acetaminophen and Its Major Metabolites in Rabbit Plasma and Urine After a Toxic Dose?
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- Author
- M.V Vertzoni; H.A Archontaki; P Galanopoulou
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0731-7085)
- Published
- 2003
- Language
- EN
- Field
- Chemistry (Physical Sciences)
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