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Identification and sequencing of 59 highly polymorphic microhaplotypes for analysis of DNA mixtures by Riga Wu; Haixia Li; Ran Li; Dan Peng; Nana Wang; Xuefeng Shen; Hongyu Sun is a scholarly article available to read on EtoBox.

What is Identification and sequencing of 59 highly polymorphic microhaplotypes for analysis of DNA mixtures about?

Mixture detection remains one of the major challenges within a forensic science context. In recent years, microhaplotypes were proposed to have great potential in mixture detection, although many of them are not as polymorphic as widely used short tandem repeat (STR) markers. In this study, 59 new highly polymorphic microhaplotypes were identified and sequenced with the NextSeq 500 Sequencer. Based on the whole 1000 Genomes Project dataset, the average effective number of alleles (Ae) of the 59 microhaplotypes was 5.44, and the Ae values of 36 of these microhaplotypes were > 5.00. Their genetic variations in 187 Han Chinese individuals were evaluated. The average allele coverage ratio (ACR) of heterozygotes across all loci was 0.96 ± 0.05. The number of observed alleles varied from 4 to 23, with an average of 8.8 alleles per microhaplotype locus. The average observed heterozygosity (Ho) of 59 loci was 0.77 ± 0.05, and the Ho values of 15 of these loci were > 0.80. All loci showed high polymorphisms with a discrimination power (DP) ranging from 0.80 to 0.97, and the average DP was 0.92 ± 0.03. The analysis of simulated mixtures demonstrated that the microhaplotypes reported here wer

Author

Riga Wu; Haixia Li; Ran Li; Dan Peng; Nana Wang; Xuefeng Shen; Hongyu Sun

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN