Can I read Increased AMPA receptor GluR1 subunit incorporation in rat hippocampal CA1 synapses during benzodiazepine withdrawal on EtoBox?

Increased AMPA receptor GluR1 subunit incorporation in rat hippocampal CA1 synapses during benzodiazepine withdrawal by Paromita Das; Scott M. Lilly; Ricardo Zerda; William T. Gunning III; Francisco J. Alvarez; Elizabeth I. Tietz is a Neuroscience article available to read on EtoBox.

What is Increased AMPA receptor GluR1 subunit incorporation in rat hippocampal CA1 synapses during benzodiazepine withdrawal about?

## Abstract Prolonged benzodiazepine treatment leads to tolerance and increases the risk of dependence. Flurazepam (FZP) withdrawal is associated with increased anxiety correlated with increased alpha‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid‐type glutamate receptor (AMPAR)‐mediated synaptic function and AMPAR binding in CA1 pyramidal neurons. Enhanced AMPAR synaptic strength is also associated with a shift toward inward rectification of synaptic currents and increased expression of GluR1, but not GluR2, subunits, suggesting augmented membrane incorporation of GluR1‐containing, GluR2‐lacking AMPARs. To test this hypothesis, the postsynaptic incorporation of GluR1 and GluR2 subunits in CA1 neurons after FZP withdrawal was examined by postembedding immunogold quantitative electron microscopy. The percentage of GluR1 positively labeled stratum radiatum (SR) synapses was significantly increased in FZP‐withdrawn rats (88.2% ± 2.2%) compared with controls (74.4% ± 1.9%). In addition, GluR1 immunogold density was significantly increased by 30% in SR synapses in CA1 neurons from FZP‐withdrawn rats compared with control rats (FZP: 14.1 ± 0.3 gold particles/μm; CON: 10.8 ± 0.4 gold

Who reads Increased AMPA receptor GluR1 subunit incorporation in rat hippocampal CA1 synapses during benzodiazepine withdrawal?

Author

Paromita Das; Scott M. Lilly; Ricardo Zerda; William T. Gunning III; Francisco J. Alvarez; Elizabeth I. Tietz

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0021-9967)

Published

2008

Language

EN

Field

Neuroscience (Life Sciences)