Can I read Control of C3b and C5b deposition by CD46 (membrane cofactor protein) after alternative but not classical complement activation on EtoBox?

Control of C3b and C5b deposition by CD46 (membrane cofactor protein) after alternative but not classical complement activation by Patricia Devaux; Dale Christiansen; Marc Fontaine; Denis Gerlier is a Immunology and Microbiology article available to read on EtoBox.

What is Control of C3b and C5b deposition by CD46 (membrane cofactor protein) after alternative but not classical complement activation about?

C3b and C5b deposition following complement activation, and its regulation by CD46 were studied using xenogenic Chinese hamster ovary (CHO) cells as targets and cytofluorometry. Following activation of the alternative pathway, an initial low level of C3b deposition was observed on CHO cell surfaces after a lag time of approximately 4 min. This was followed by a secondary high level of C3b deposition with a slower rate. C3b deposition was maximal within 15 min. When CD46 was expressed (B2 isoform), the kinetics of C3b deposition were essentially unchanged, but the onset of the secondary high C3b deposition was fully prevented. C5b deposition was also observed on CHO but not on CHO.CD46 cells following activation of the alternative pathway. Activation of the classical pathway on CHO and CHO.CD46 cells, using factor B-depleted human serum and anti-CHO antibodies, resulted in almost identical single-peak C3b deposition profiles. Accordingly, no regulation of C5b deposition by CD46 was evident following activation of the classical pathway. These data indicate that CD46 prevents the C3b deposition amplification loop mediated by the alternative C3 convertase and, consequently, inhibits th

Who reads Control of C3b and C5b deposition by CD46 (membrane cofactor protein) after alternative but not classical complement activation?

Author

Patricia Devaux; Dale Christiansen; Marc Fontaine; Denis Gerlier

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 0014-2980)

Published

1999

Language

EN

Field

Immunology and Microbiology (Life Sciences)