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DCA Effects on Cancer Stem Cells Analysis is a document available to read on EtoBox.
The document analyzes the effects of dichloroacetate (DCA) on normal and cancerous stem cells. Methods included culturing cells, examining gene expression through RT-PCR, testing cell viability with Trypan Blue, and applying various concentrations of DCA. Results showed that increased DCA concentration decreased proliferation in cancerous K562 cells but not in normal stem cells. DCA also inhibited expression of the WT1 gene, which controls cell proliferation, in K562 cells treated with 20mM DCA. The conclus
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