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Long Noncoding RNA X-Inactive Specific Transcript Facilitates Cellular Functions in Melanoma via miR-139-5p/ROCK1 Pathway by Tian, Ke; Sun, Dongxia; Chen, Min; Yang, Yifei; Wang, Fang; Guo, Taotao; Shi, Zhimin is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Long Noncoding RNA X-Inactive Specific Transcript Facilitates Cellular Functions in Melanoma via miR-139-5p/ROCK1 Pathway about?

## Purpose Although X-inactive specific transcript (XIST) is known to play a critical role in the pathogenesis of melanoma, the mechanisms through which this remains unclear. ## Methods RNAseq, immunohistochemistry, and qRT-PCR were used to identify the levels of XIST, miR-139-5p, and Rho-Associated Coiled-Coil Containing Protein Kinase-1 (ROCK1) in melanoma tissues and cells. A subcellular fractionation assay was used to determine the location of XIST. CCK-8 and colony formation assays were used to evaluate cellular proliferation. Cell migration and wound healing assays were used to detect the effects on cell migration. RNA pull-down was used to confirm the interaction between XIST and miR-139-5p. Besides, the xenograft tumor experiment was performed to further verify the roles of XIST in melanoma. ## Results In this study, an increased level of XIST was revealed in melanoma tissues and cells, which was associated with higher TNM stage and positive lymph node metastasis. XIST was found to function as a “molecular sponge” of miR-139-5p to facilitate cellular functions. Moreover, these consequences could be partially reversed by inhibition of miR-139-5p. MiR-139-5p was found to targ

Who reads Long Noncoding RNA X-Inactive Specific Transcript Facilitates Cellular Functions in Melanoma via miR-139-5p/ROCK1 Pathway?

Author

Tian, Ke; Sun, Dongxia; Chen, Min; Yang, Yifei; Wang, Fang; Guo, Taotao; Shi, Zhimin

Publisher

Dove Press Ltd.; Dove Medical Press; Dove Medical Press Ltd; [Auckland N.Z.]: Dove Medical Press, c2008-; Informa UK Limited; Dove Medical Press Ltd. (ISSN 1178-6930)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)