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This document describes a study that established an isotachophoretic (ITP) method to analyze metabolites of glucose pathways in human erythrocytes. The method allows determination of the main compounds of glycolysis, including ATP, ADP, lactate, pyruvate, and 2,3-diphosphoglycerate, in a single run. Erythrocytes were isolated from blood and incubated with different glucose concentrations to mimic situations like diabetes. ITP was used to separate and characterize the metabolites. Calibration curves were dev

Author

Eka Herlina

Language

EN