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Sub-MIC vancomycin enhances the antibiotic tolerance of vancomycin-intermediate Staphylococcus aureus through downregulation of protein succinylation by Yi Yang; Li Tan; Siyuan He; Bo Hao; Xiaonan Huang; Yumin Zhou; Weilong Shang; Huagang Peng; Zhen Hu; Ruolan Ding; Xiancai Rao is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Sub-MIC vancomycin enhances the antibiotic tolerance of vancomycin-intermediate Staphylococcus aureus through downregulation of protein succinylation about?
Bacteria develop tolerance after transient exposure to antibiotics, and tolerance is a significant driver of resistance. The purpose of this study is to evaluate the mechanisms underlying tolerance formation in vancomycinintermediate Staphylococcus aureus (VISA) strains. VISA strains were cultured with sub-minimum inhibitory concentrations (sub-MICs) of vancomycin. Enhanced vancomycin tolerance was observed in VISA strains with distinct genetic lineages. Western blot revealed that the VISA protein succinylation (Ksucc) levels decreased with the increase in vancomycin exposure. Importantly, Ksucc modification, vancomycin tolerance, and cell wall synthesis were simultaneously affected after deletion of SacobB, which encodes a desuccinylase in S. aureus. Several Ksucc sites were identified in MurA, and vancomycin MIC levels of murA mutant and Ksucc-simulated (MurA(K69E) and MurA(K191E)) mutants were reduced. The vancomycin MIC levels of K65-MurA(K191E) in particular decreased to 1 mg/L, converting VISA strain K65 to a vancomycin-susceptible S. aureus strain. We further demonstrated that the enzymatic activity of MurA was dependent on Ksucc modification. Our data suggested the influenc
Who reads Sub-MIC vancomycin enhances the antibiotic tolerance of vancomycin-intermediate Staphylococcus aureus through downregulation of protein succinylation?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Yi Yang; Li Tan; Siyuan He; Bo Hao; Xiaonan Huang; Yumin Zhou; Weilong Shang; Huagang Peng; Zhen Hu; Ruolan Ding; Xiancai Rao
- Publisher
- Elsevier BV
- Published
- 2024
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)