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Comparison of extraction methods of phytochemical compounds from white flower variety of Melastoma malabathricum 1 1. Introduction 1 2. Materials and methods 2 2.1. Chemicals and reagents 2 2.2. Preparation of M. malabathricum 2 2.3. Preparation and comparison of extraction method 2 2.3.1. Conventional Soxhlet Extraction (CSE) method 2 2.3.2. Modified Ultrasound-Assisted Extraction (MUAE) method 2 2.3.3. Ultrasound-Assisted Extraction (UAE) method 3 2.4. Phytochemical content analysis 3 2.4.1. Analysis of total flavonoid content (TFC) 3 2.4.2. Analysis of total phenolic content (TPC) 3 2.4.3. Analysis of total saponin content 3 2.4.4. Analysis of total polysaccharides content 3 2.4.5. Analysis of total protein content 3 2.5. DPPH free radical scavenging activity analysis of the extracts 3 2.6. Metabolite profiles determination by UHPLC-Q-Orbitrap-MS/MS 4 2.7. Statistical analysis 4 3. Results and discussion 4 3.1. Characterization of phytochemical contents from M. malabathricum 4 3.2. Comparison of the phytochemical yields using CSE, UAE and MUAE methods 5 3.3. Antioxidant activity by DPPH free radical scavenging 5 3.4. Correlation between phytochemical content and antioxidant acti

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Language

UND

Category

nonfiction