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Can I read Streptavidin Chiral Stationary Phase for the Separation of Adenosine Enantiomers on EtoBox?
Streptavidin Chiral Stationary Phase for the Separation of Adenosine Enantiomers by Corinne Ravelet; Michael Michaud; Anne Ravel; Catherine Grosset; Annick Villet; Eric Peyrin is a Chemistry article available to read on EtoBox.
What is Streptavidin Chiral Stationary Phase for the Separation of Adenosine Enantiomers about?
In this paper, a microbore column packed with streptavidin particles was used, at various temperatures (0-24 • C), to separate the adenosine enantiomers by HPLC. Using an aqueous mobile phase, the apparent enantioseparation was high for a small molecule, varying from 11.5 at 0 • C to 6.2 at 24 • C. From the experiments carried out with a streptavidin-biotin complex stationary phase, it was demonstrated that the blockage of the biotin sites of the immobilized streptavidin was responsible for a strong decrease in the enantioselectivity via a direct and/or an indirect effect. From the analysis of the concentration dependencies of the solute retention factor, it was also shown that a reduction of the d-adenosine specific binding sites occurred at the lowest temperature. The thermodynamic parameters determined from the van't Hoff plots indicated that the d-adenosine binding to the streptavidin specific sites was enthalpically driven.
Who reads Streptavidin Chiral Stationary Phase for the Separation of Adenosine Enantiomers?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Corinne Ravelet; Michael Michaud; Anne Ravel; Catherine Grosset; Annick Villet; Eric Peyrin
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 2004
- Language
- EN
- Field
- Chemistry (Physical Sciences)