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Concentrating Rotaviruses from Water Samples Using Monolithic Chromatographic Supports by Ion Gutiérrez-Aguirre; Marko Banjac; Andrej Steyer; Mateja Poljšak-Prijatelj; Matjaž Peterka; Aleš Štrancar; Maja Ravnikar is a Chemistry article available to read on EtoBox.
Rotaviruses are the leading cause of diarrhoea in infants around the globe and, under certain conditions they can be present in drinking water sources and systems. Ingestion of 10-100 viral particles is enough to cause disease, emphasizing the need for sensitive diagnostic methods. In this study we have optimized the concentration of rotavirus particles using methacrylate monolithic chromatographic supports. Different surface chemistries and mobile phases were tested. A strong anion exchanger and phosphate buffer (pH 7) resulted in the highest recoveries after elution of the bound virus with 1 M NaCl. Using this approach, rotavirus particles spiked in 1 l volumes of tap or river water were efficiently concentrated. The developed concentration method in combination with a real time quantitative polymerase chain reaction assay detected rotavirus concentrations as low as 100 rotavirus particles/ml.
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- Author
- Ion Gutiérrez-Aguirre; Marko Banjac; Andrej Steyer; Mateja Poljšak-Prijatelj; Matjaž Peterka; Aleš Štrancar; Maja Ravnikar
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 2009
- Language
- EN
- Field
- Chemistry (Physical Sciences)