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Can I read Characterization and Optimization of Fluorescent Organosilica Colloids for 3D Confocal Microscopy Prepared Under “Zero-Flow” Conditions on EtoBox?
Characterization and Optimization of Fluorescent Organosilica Colloids for 3D Confocal Microscopy Prepared Under “Zero-Flow” Conditions by Ruth A. Crothers; Nicholas H. P. Orr; Berend van der Meer; Roel P. A. Dullens; Taiki Yanagishima is a Materials Science article available to read on EtoBox.
What is Characterization and Optimization of Fluorescent Organosilica Colloids for 3D Confocal Microscopy Prepared Under “Zero-Flow” Conditions about?
We optimize and characterize the preparation of 3-trimethoxysilyl propyl methacrylate (TPM) colloidal suspensions for three-dimensional confocal microscopy. We revisit a simple synthesis of TPM microspheres by nucleation of droplets from prehydrolyzed TPM oil in a "zero-flow" regime and demonstrate how precise and reproducible control of particle size may be achieved via singlestep nucleation with a focus on how the reagents are mixed. We also revamp the conventional dyeing method for TPM particles to achieve uniform transfer of a fluorophore to the organosilica droplets, improving particle identification. Finally, we illustrate how a ternary mixture of tetralin, trichloroethylene, and tetrachloroethylene may be used as a suspension medium which matches the refractive index of these particles while allowing independent control of the density mismatch between particle and solvent.
Who reads Characterization and Optimization of Fluorescent Organosilica Colloids for 3D Confocal Microscopy Prepared Under “Zero-Flow” Conditions?
It is typically read by researchers, students, and practitioners in Materials Science.
- Author
- Ruth A. Crothers; Nicholas H. P. Orr; Berend van der Meer; Roel P. A. Dullens; Taiki Yanagishima
- Publisher
- American Chemical Society (ACS)
- Published
- 2023
- Language
- EN
- Field
- Materials Science (Physical Sciences)