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Characterization of a Brassica napus gene encoding a cruciferin subunit: estimation of sizes of cruciferin gene families by Joakim Rdin; Staffan Sjdahl; Lars-Gran Josefsson; Lars Rask is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Characterization of a Brassica napus gene encoding a cruciferin subunit: estimation of sizes of cruciferin gene families about?

A gene encoding a subunit of the 12S storage globulin, cruciferin, in Brassica napus (oilseed rape) has been isolated and characterized. The gene consists of about 2200 bp including three short intervening sequences. Primer extension analysis showed that the major transcription start site is located 30 bp 5' of the predicted ATG start codon. This gene belongs to one of three different major families encoding cruciferin subunits. By use of gene-family-specific probes and Southern blotting analysis the number of genes of the three different cruciferin subtypes in B. napus was estimated.

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Author

Joakim Rdin; Staffan Sjdahl; Lars-Gran Josefsson; Lars Rask

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0167-4412)

Published

1992

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)