About this Biochemistry, Genetics and Molecular Biology article

The effect of ArcA on the growth, motility, biofilm formation, and virulence of Plesiomonas shigelloides by Junxiang Yan; Yuehua Li; Xueqian Guo; Xiaochen Wang; Fenxia Liu; Ang Li; Boyang Cao is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

## Abstract ## Background The anoxic redox control binary system plays an important role in the response to oxygen as a signal in the environment. In particular, phosphorylated ArcA, as a global transcription factor, binds to the promoter regions of its target genes to regulate the expression of aerobic and anaerobic metabolism genes. However, the function of ArcA in Plesiomonas shigelloides is unknown. ## Results In the present study, P. shigelloides was used as the research object. The differences in growth, motility, biofilm formation, and virulence between the WT strain and the ΔarcA isogenic deletion mutant strain were compared. The data showed that the absence of arcA not only caused growth retardation of P. shigelloides in the log phase, but also greatly reduced the glucose utilization in M9 medium before the stationary phase. The motility of the ΔarcA mutant strain was either greatly reduced when grown in swim agar, or basically lost when grown in swarm agar. The electrophoretic mobility shift assay results showed that ArcA bound to the promoter regions of the flaK, rpoN, and cheV genes, indicating that ArcA directly regulates the expression of these three motility-related

Author

Junxiang Yan; Yuehua Li; Xueqian Guo; Xiaochen Wang; Fenxia Liu; Ang Li; Boyang Cao

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)