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Barley aleurone cell development: molecular cloning of aleurone-specific cDNAs from immature grains by Kjetill Jakobsen; Sonja Sletner Klemsdal; Reidunn B. Aalen; Marie Bosnes; Danny Alexander; Odd-Arne Olsen is a Agricultural and Biological Sciences article available to read on EtoBox.

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The cloning of 11 different homology groups of cDNAs representing genes expressed in aleurone, but not in starchy endosperm of 20-day-old barley grains is described. Among the cDNAs, four are aleurone-specific, while the remaining are also expressed in the embryo, but not in any other part of the plant. Sequence analysis of one of the aleurone-specific clones, BllE, reveals an open reading frame coding for an unidentified 10.4 kDa protein with a putative signal sequence and a possible metal-binding finger. The BllE gene has a high GC content in the 5' leader sequence (63%), as well as in the coding region (70%) compared to known cDNAs from the barley starchy endosperm. Northern analysis of BllE indicates maximum mRNA abundance around mid-phase of grain development. When isolated immature aleurone/pericarp is incubated in tissue culture medium (MS) the BllE message disappears, indicating a requirement for a diffusible factor from the intact grain for its continued presence.

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Author

Kjetill Jakobsen; Sonja Sletner Klemsdal; Reidunn B. Aalen; Marie Bosnes; Danny Alexander; Odd-Arne Olsen

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0167-4412)

Published

1989

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)