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Ion currents involved in early Nod factor response in Medicago sativa root hairs: a discontinuous single-electrode voltage-clamp study by Armen Kurkdjian; François Bouteau; Anne-Marie Pennarun; Monique Convert; Daniel Cornel; Jean-Pierre Rona; Ulrike Bousquet is a Agricultural and Biological Sciences article available to read on EtoBox.

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## Summary Nod factor [NodRm‐IV(Ac,S)], isolated from the bacterium __Rhizobium meliloti__, induces a well‐known depolarization in __Medicago sativa__ (cv Sitel) root hairs. Analysis of this membrane response using the discontinuous single‐electrode voltage‐clamp technique (dSEVC) shows that anion channel, K^+^ channel and H^+^‐ATPase pump currents are involved in young growing root hairs. The early Nod‐factor‐induced depolarization is due to increase of the inward ion current and inhibition of the H^+^ pump. It involved an instantaneous inward anion current (IIAC) and/or a time‐dependent inward K^+^ current (IRKC). These two ion currents are then down‐regulated while the H^+^ pump is stimulated, allowing long‐term rectification of the membrane potential (__E__~m~). Our results support the idea that the regulation of inward current plays a primary role in the Nod‐factor‐induced electrical response, the nature of the ions carried by these currents depending on the activated anion and/or K^+^ channels at the plasma membrane.

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Author

Armen Kurkdjian; François Bouteau; Anne-Marie Pennarun; Monique Convert; Daniel Cornel; Jean-Pierre Rona; Ulrike Bousquet

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)

Published

2000

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)