Can I read HPLC–DAD determination of opioids, cocaine and their metabolites in plasma on EtoBox?

HPLC–DAD determination of opioids, cocaine and their metabolites in plasma by P. Fernández; L. Morales; C. Vázquez; A.M. Bermejo; M.J. Tabernero is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is HPLC–DAD determination of opioids, cocaine and their metabolites in plasma about?

High performance liquid chromatography with diode array detection (HPLC-DAD) was used to develop a method for the simultaneous determination of morphine, codeine, 6-acetylmorphine (6AM), cocaine, benzoylecgonine (BEG), cocaethylene, methadone and its metabolite, 2-ethylidene-1,5-dimethyldiphenylpyrrolidine (EDDP), in plasma. Following solid-phase extraction with Bond Elut Certify cartridges, chromatography was performed on an X-Terra 1 RP8 column (250 mm  4.6 mm i.d., 5 mm particle size), using acetonitrile-phosphate buffer pH 6.53 as mobile phase and elution in the gradient mode. The detector response was linear at concentrations over the range 0.1-10 mg/mL in plasma, and the correlation coefficients for the eight drugs studied were all higher than 0.99. The average extraction recoveries from plasma ranged from 60% for BEG to 95% for methadone. The precision was acceptable, with coefficients of variation oscillating between 2.55% and 6.45%. The accuracy was found to be within satisfactory limits (AE8.1%). Finally, the method was applied to 21 plasma samples from fatal overdoses, obtaining positive results for two or more drugs.

Who reads HPLC–DAD determination of opioids, cocaine and their metabolites in plasma?

Author

P. Fernández; L. Morales; C. Vázquez; A.M. Bermejo; M.J. Tabernero

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0379-0738)

Published

2006

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)