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An Intrinsic Control Element for Translational Initiation in Class 1 Integrons by Béatrice Hanau‐Berçot; Isabelle Podglajen; Isabelle Casin; Ekkehard Collatz is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Summary Integrons are genetic elements able to capture anti‐biotic resistance and other genes and to promote their transcription. Here, we have investigated integron‐dependent translation of an aminoglycoside 6**′**‐N‐acetyltransferase gene (__aac(6__**′**__)‐Ib~7~__) inserted at the __attI1__ site. N‐terminal sequencing revealed that translation of this gene was initiated at a GTG codon, which is not part of a plausible translation initiation region (TIR). A short open reading frame (called ORF‐11) overlapping the __attI1__ site was probed by site‐directed mutagenesis for its contribution to __aac(6__**′**__)‐Ib~7~__ translation. When ORF‐11 and its TIR were deleted __en bloc__, translational efficiency dropped by over 80%, as determined with an acetyltransferase– luciferase fusion product. Invalidation of the ATG start codon of ORF‐11 or its putative Shine–Dalgarno sequence resulted in a decrease of over 60%, whereas the decrease was much less pronounced when the amino acid sequence of the putative ORF‐11‐encoded peptide was altered or when the distance between ORF‐11 and __aac(6__**′**__)‐Ib~7~__ was doubled. This demonstrates that __aac(6__**′**__)‐Ib~7~__ translation is dep

Who reads An Intrinsic Control Element for Translational Initiation in Class 1 Integrons?

Author

Béatrice Hanau‐Berçot; Isabelle Podglajen; Isabelle Casin; Ekkehard Collatz

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)

Published

2002

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)