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Sulfactin, A New Antibiotic Substance Produced By A Soil Actinomyces by Renate Junowicz-Kocholaty; Walter Kocholaty; Albert Kelner is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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We have isolated in crystalline form an antibiotic substance from the culture filtrate of an Actinomyces (R-30) found by one of us (R. J.-K.) in non-manured soil. It is active against certain Gram-positive organisms and inactive against Gram-negative bacteria. The Actinomyces, designated as R-30, was studied by one of us (A. K.) and found to resemble Actinomyces roseus in some respects. It is impossible to state whether it is a variant of Actinomyces roseus or a new species. No true spirals were seen but some aerial hyphae formed loops. Conidia are large, spherical, and borne in chains. The optimum temperature for growth is about 25"; growth takes place at 20" and 37" but not at 15". Peptone and gelatin media are not blackened. A faint pink soluble pigment is produced in calcium citrate, glucose-asparagine, and Czapek agar containing glucose instead of sucrose. No soluble pigment was formed in other media studied. There was abundant gray-buff growth on nutrient agar; sporulation was moderate, white, becoming faint pink. Moderate gray growth was observed on glucose-asparagine agar with bright pink spores. Growth is scanty, with pink spores, on Czapek agar and glucose-nitrate agar. T

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Author

Renate Junowicz-Kocholaty; Walter Kocholaty; Albert Kelner

Publisher

Elsevier BV

Published

1947

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)