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Free Docosahexaenoic Acid Promotes Ferroptotic Cell Death via Lipoxygenase Dependent and Independent Pathways in Cancer Cells by Kai Shan; Ninghan Feng; Doudou Zhu; Hongyan Qu; Guoling Fu; Jiaqi Li; Jing Cui; Heyan Chen; Rong Wang; Yumin Qi; Yong Q. Chen is a Medicine article available to read on EtoBox.
What is Free Docosahexaenoic Acid Promotes Ferroptotic Cell Death via Lipoxygenase Dependent and Independent Pathways in Cancer Cells about?
## Purpose: Ferroptosis is a form of regulated cell death that has the potential to be targeted as a cancer therapeutic strategy. but cancer cells have a wide range of sensitivities to ferroptosis, which limits its therapeutic potential. accumulation of lipid peroxides determines the occurrence of ferroptosis. however, the type of lipid involved in peroxidation and the mechanism of lipid peroxide accumulation are less studied. ## Methods: The effects of fatty acids (10 μm) with different carbon chain length and unsaturation on ferroptosis were evaluated by mtt and ldh release assay in cell lines derived from prostate cancer (pc3, 22rv1, du145 and lncap), colorectal cancer (ht-29), cervical cancer (hela) and liver cancer (hepg2). inhibitors of apoptosis, necroptosis, autophagy and ferroptosis were used to determine the type of cell death. then the regulation of reactive oxygen species (ros) and lipid peroxidation by docosahexaenoic acid (dha) was measured by hplc-ms and flow cytometry. the avtive form of dha was determined by sirna mediated gene silencing. the role of lipoxygenases was checked by inhibitors and gene silencing. finally, the effect of dha on ferroptosis-mediated tumor
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- Author
- Kai Shan; Ninghan Feng; Doudou Zhu; Hongyan Qu; Guoling Fu; Jiaqi Li; Jing Cui; Heyan Chen; Rong Wang; Yumin Qi; Yong Q. Chen
- Publisher
- Springer Science and Business Media LLC
- Published
- 2022
- Language
- EN
- Field
- Medicine (Health Sciences)