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Correlation of the Physicochemical Properties of Symmetric 1,3-Dialkoylamidopropane-based Cationic Lipids Containing Single Primary and Tertiary Amine Polar Head Groups with in Vitro Transfection Activity by Michalakis Savva; Ahmad Aljaberi; Jennifer Feig; Donna Beer Stolz is a Materials Science article available to read on EtoBox.

What is Correlation of the Physicochemical Properties of Symmetric 1,3-Dialkoylamidopropane-based Cationic Lipids Containing Single Primary and Tertiary Amine Polar Head Groups with in Vitro Transfection Activity about?

The physicochemical properties of a novel series of symmetric 1,3-dialkylamidopropane-based cationic amphiphiles [M. Sheikh, J. Feig, B. Gee, S. Li, M. Savva, In vitro lipofection with novel series of symmetric 1,3-dialkoylamidopropane-based cationic surfactants containing single primary and tertiary amine polar head groups, Chem. Phys. Lipids 124 (2003) 49-61] were studied by several techniques, in an effort to correlate cationic lipid structure with transfection efficacy. It was found that only the unsubstituted amine and tertiary amine dioleoyl derivatives 1,3lmp5 and 1,3lmt5, respectively, mediated in vitro transfection activity in the absence of helper lipids. This activity pattern was consistent with ethidium bromide fluorescence quenching studies, which indicated that only these two derivatives bound to and efficiently condense plasmid DNA at physiological pH. Dynamic light scattering indicated that lipoplexes made by these two cationic lipids were relatively small particles below 1 microm, in sharp contrast to lipoplexes bigger than 3 microm composed of saturated cationic derivatives. Transmission electron microscopy studies clearly indicated that cationic lipid dispersions

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Author

Michalakis Savva; Ahmad Aljaberi; Jennifer Feig; Donna Beer Stolz

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0927-7765)

Published

2005

Language

EN

Field

Materials Science (Physical Sciences)