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Reduction of liver tumor necrosis factor-α expression by targeting delivery of antisense oligonucleotides into Kupffer cells protects rats from fulminant hepatitis by Lei Dong; Longsheng Zuo; Suhua Xia; Shuying Gao; Chenyu Zhang; Jiangning Chen; Junfeng Zhang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Reduction of liver tumor necrosis factor-α expression by targeting delivery of antisense oligonucleotides into Kupffer cells protects rats from fulminant hepatitis about?
## Abstract ## Background Fulminant liver failure can cause extreme mortality due to the lack of effective and targeting therapeutics for the disease. Novel therapeutics using antisense technology require an efficient and safe delivery system with Kupffer cell targeting ability. ## Methods We explored the capacity of galactosylated low molecular weight chitosan (GLC) to efficiently mediate the antisense oligonucleotide (ASO) TJU‐2755 into Kupffer cells, enhance the effect of the oligonucleotides on the suppression of tumor necrosis factor (TNF)‐α and prolong the active time of the antisense drug __in vivo__. The protective and therapeutic effect of ASO/GLC in the animal model of D‐galactosamine/lipopolysaccharide‐induced fulminant hepatitis was tested. ## Results ASOs delivered by GLC were concentrated in Kupffer cells and more potent in reducing the expression of TNF‐α mRNA, as well as reducing serum TNF‐α levels. Furthermore, the ASO/GLC complex successfully rescued animals from fulminant hepatitis and mortality. Compared to naked ASO, the complex notably reduced the dose administrated in animals and prolonged its effectiveness. A single dose of 5 mg ASO per kg body weight achiev
Who reads Reduction of liver tumor necrosis factor-α expression by targeting delivery of antisense oligonucleotides into Kupffer cells protects rats from fulminant hepatitis?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Lei Dong; Longsheng Zuo; Suhua Xia; Shuying Gao; Chenyu Zhang; Jiangning Chen; Junfeng Zhang
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1099-498X)
- Published
- 2009
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)