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Na^+^/K^+^/Cl^−^ cotransporter activates mitogen‐activated protein kinase in fibroblasts and lymphocytes by Rivka Panet; Michal Eliash; Marjory Pick; Henri Atlan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract In a previous work, we have shown that overexpression of the Na^+^/K^+^/Cl^−^ cotransporter (NKCC1) induces cell proliferation and transformation. We investigate in the present study the role of the NKCC1 in the mitogenic signal transduction. We show that overexpression of the cotransporter gene (NKCC1) in stablely transfected cells (Balb/c‐NKCC1), resulted in enhanced phosphorylation of the extracellular regulated kinase (ERK) to produce double phosphorylated ERK (DP‐ERK). Furthermore, the level of DP‐ERK was reduced by 50–80% following the addition of bumetanide, a specific inhibitor of the Na^+^/K^+^/Cl^−^ cotransporter, in quiescent as well as in proliferating cultures of the Balb/c‐NKCC1 clone. In order to explore further the role of the Na^+^/K^+^/Cl^−^ cotransporter in mitogenic signal transduction, we measured the effect of the two specific inhibitors of the cotransporter; bumetanide and furosemide, on DP‐ERK level in immortalized non‐transformed cells. In Balb/c 3T3 fibroblasts stimulated with FGF, bumetanide, and furosemide inhibited 50–60% of the ERK 1/2 phosphorylation. The inhibitor concentration needed for maximal inhibition of ERK 1/2 phosphorylation was

Who reads Na^+^/K^+^/Cl^−^ cotransporter activates mitogen‐activated protein kinase in fibroblasts and lymphocytes?

Author

Rivka Panet; Michal Eliash; Marjory Pick; Henri Atlan

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

2002

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)