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Plasmodium falciparum: PCR detection and genotyping of isolates from peripheral, placenta), and cord blood of pregnant Malawian women and their infants by Deborah D. Kamwendo; Fraction K. Dzinjalamala; Georges Snounou; Maxwell C.C. Kanjala; Chisale G. Mhango; Malcolm E. Molyneux; Stephen J. Rogerson is a Medicine article available to read on EtoBox.
What is Plasmodium falciparum: PCR detection and genotyping of isolates from peripheral, placenta), and cord blood of pregnant Malawian women and their infants about?
Polymerase chain reaction (PCR) is both a sensitive means of detecting malaria parasitaemia, and a simple tool for identifying genetic differences in parasites infecting human subjects. We compared PCR to microscopy in detection of Plasmodium falciparum infection in peripheral, placental and cord blood samples collected from 13 1 pregnant Malawian women and their infants in 1997-99. Infections detected by species-specific PCR were genotyped at the merozoite surface protein 1 and 2 loci, and minimum numbers of infecting genotypes determined. PCR was of similar sensitivity to microscopy in detecting peripheral and placental infection, and placental blood PCR was 100% specific compared to placental histology. Cord blood parasitaemia was more frequently detected by PCR than microscopy, 20% versus 6%. Genotype numbers in peripheral blood (mean 2.36; range l-5), placental blood (mean 2.41; range l-6) and cord (mean 2.14; range l-4) were similar. The frequency of detection of each allelic family did not differ between sites. Genotypes from different sites in each patient were compared. In 69% of women, genotypes were detected in peripheral blood and not placenta, or vice versa, suggesting
Who reads Plasmodium falciparum: PCR detection and genotyping of isolates from peripheral, placenta), and cord blood of pregnant Malawian women and their infants?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Deborah D. Kamwendo; Fraction K. Dzinjalamala; Georges Snounou; Maxwell C.C. Kanjala; Chisale G. Mhango; Malcolm E. Molyneux; Stephen J. Rogerson
- Publisher
- Elsevier Science; Oxford University Press; Oxford University Press (OUP); ScienceOpen (ISSN 0035-9203)
- Published
- 2002
- Language
- EN
- Field
- Medicine (Health Sciences)