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Bone Morphogenetic Protein-4 Regulates Its Own Expression in Cultured Osteoblasts by Renata C. Pereira; Sheila Rydziel; Ernesto Canalis is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Bone Morphogenetic Protein-4 Regulates Its Own Expression in Cultured Osteoblasts about?

During development, bone morphogenetic proteins (BMPs) induce the differentiation of mesenchymal progenitor cells to enter into the osteoblastic lineage, and BMPs enhance osteoblastic function. BMPs and noggin, a specific binding protein that blocks BMP actions, are expressed by osteoblastic cells but there is limited information about regulation of BMP synthesis in skeletal cells. We tested for the expression and regulation of BMP-4 in cultures of osteoblast-enriched cells from 22-day fetal rat calvariae (Ob cells). BMP-4 caused a short-lived increase in BMP-4 mRNA followed by a marked inhibition of BMP-4 expression. The stimulatory effect was transcriptional, as determined by nuclear run-on assays, whereas the inhibitory effect was transcriptional and posttranscriptional, because longer BMP-4 exposure decreased its rate of transcription and shortened the half-life of BMP-4 mRNA in transcriptionally arrested Ob cells. BMP-2 and BMP-6 also inhibited BMP-4 mRNA levels. Transforming growth factor ␤1 increased, whereas fibroblast growth factor-2, platelet-derived growth factor BB, and insulinlike growth factor I decreased BMP-4 mRNA in Ob cells. BMP-2 also was expressed by Ob cells an

Who reads Bone Morphogenetic Protein-4 Regulates Its Own Expression in Cultured Osteoblasts?

Author

Renata C. Pereira; Sheila Rydziel; Ernesto Canalis

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)