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Can I read Translation of vph mRNA in Streptomyces lividans and Escherichia coli after removal of the 5′ untranslated leader on EtoBox?
Translation of vph mRNA in Streptomyces lividans and Escherichia coli after removal of the 5′ untranslated leader by Chi-Ju Wu; Gary R. Janssen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Translation of vph mRNA in Streptomyces lividans and Escherichia coli after removal of the 5′ untranslated leader about?
The __Streptomyces vinaceus__ viomycin phosphotransferase (__vph__) mRNA contains an untranslated leader with a conventional Shine–Dalgarno homology. The __vph__ leader was removed by ligation of the __vph__ coding sequence to the transcriptional start site of a __Strepto‐myces__ or an __Escherichia coli__ promoter, such that transcription would initiate at the first position of the __vph__ start codon. Analysis of mRNA demonstrated that transcription initiated primarily at the A of the __vph__ AUG translational start codon in both __Streptomyces lividans__ and __E. coli__; cells expressing the unleadered __vph__ mRNA were resistant to viomycin indicating that the Shine–Dalgarno sequence, or other features contained within the leader, was not necessary for __vph__ translation. Addition of four nucleotides (5′‐AUGC‐3′) onto the 5′ end of the unleadered __vph__ mRNA resulted in translation initiation from the __vph__ start codon and the AUG triplet contained within the added sequence. Translational fusions of __vph__ sequence to a Tn__5 neo__ reporter gene indicated that the first 16 codons of __vph__ coding sequence were sufficient to specify the translational start site and reading
Who reads Translation of vph mRNA in Streptomyces lividans and Escherichia coli after removal of the 5′ untranslated leader?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Chi-Ju Wu; Gary R. Janssen
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)
- Published
- 1996
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)