Can I read Temperature dependence of the preferential interactions of ribonuclease a in aqueous co‐solvent systems: Thermodynamic analysis on EtoBox?
Temperature dependence of the preferential interactions of ribonuclease a in aqueous co‐solvent systems: Thermodynamic analysis by Guifu Xie; Serge N. Timasheff is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Temperature dependence of the preferential interactions of ribonuclease a in aqueous co‐solvent systems: Thermodynamic analysis about?
## Abstract The temperature dependence of preferential solvent interactions with ribonuclease A in aqueous solutions of 30% sorbitol, 0.6 M MgCl~2~, and 0.6 M MgSO~4~ at low pH (1.5 and 2.0) and high pH (5.5) has been investigated. This protein was stabilized by all three co‐solvents, more so at low pH than high pH (except 0.6 M MgCl~2~ at pH 5.5). The preferential hydration of protein in all three co‐solvents was high at temperatures below 30 °C and decreased with a further increase in temperature (for 0.6 M MgCl~2~ at pH 5.5, this was not significant), indicating a greater thermodynamic instability at low temperature than at high temperature. The preferential hydration of denatured protein (low pH, high temperature) was always greater than that of native protein (high pH, high temperature). In 30% sorbitol, the interaction passed to preferential binding at 45% for native ribonuclease A and at 55 °C for the denatured protein. Availability of the temperature dependence of the variation with sorbitol concentration of the chemical potential of the protein, (__∂μ~2~/∂m__~3~__)~T, P, m2~__, permitted calculation of the corresponding enthalpy and entropy parameters. Combination with ava
Who reads Temperature dependence of the preferential interactions of ribonuclease a in aqueous co‐solvent systems: Thermodynamic analysis?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Guifu Xie; Serge N. Timasheff
- Publisher
- Cold Spring Harbor Laboratory Press; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0961-8368)
- Published
- 1997
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)