Can I read MiR‐216b inhibits osteosarcoma cell proliferation, migration, and invasion by targeting Forkhead Box M1 on EtoBox?

MiR‐216b inhibits osteosarcoma cell proliferation, migration, and invasion by targeting Forkhead Box M1 by Wei Wang; Zijun Guo; Hong Yu; Ling Fan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is MiR‐216b inhibits osteosarcoma cell proliferation, migration, and invasion by targeting Forkhead Box M1 about?

## Abstract Osteosarcoma (OS) is considered the most common type of primary malignant bone tumor, which has a high rate of mortality in children and adolescents. However, the current treatment methods for OS are ineffective. Therefore, there is an urgent requirement to identify the critical targets. This study aimed to identify the roles and significance of microRNA‐216b (miR‐216b) in OS. To explore the cellular and molecular functions of miR‐216b and Forkhead Box M1 (FoxM1) in OS, the expression of miR‐216b and FoxM1 at the transcriptional level was measured using quantitative real‐time PCR (qRT‐PCR). Wound healing assay, 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5 diphenyltetrazolium bromide assay (MTT) assay, flow cytometry, and transwell invasion assay were conducted to study the function of miR‐216b and FoxM1 in OS cells. Dual luciferase reporter assay was performed to identify the relationships between miR‐216b and FoxM1. qRT‐PCR results revealed that miR‐216b expression was significantly downregulated, and FoxM1 was observed to be significantly upregulated in human OS cell lines (MG‐63) and tissues. MTT data showed that upregulation of miR‐216b expression led to cell growth inhibition

Who reads MiR‐216b inhibits osteosarcoma cell proliferation, migration, and invasion by targeting Forkhead Box M1?

Author

Wei Wang; Zijun Guo; Hong Yu; Ling Fan

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2018

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)