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Can I read Development of molecular assays for the identification of the 11 Eimeria species of the domestic rabbit (Oryctolagus cuniculus) on EtoBox?
Development of molecular assays for the identification of the 11 Eimeria species of the domestic rabbit (Oryctolagus cuniculus) by Ursula C. Oliveira; Jane S. Fraga; Dominique Licois; Michal Pakandl; Arthur Gruber is a Immunology and Microbiology article available to read on EtoBox.
What is Development of molecular assays for the identification of the 11 Eimeria species of the domestic rabbit (Oryctolagus cuniculus) about?
Coccidiosis are the major parasitic diseases in poultry and other domestic animals including the domestic rabbit (Oryctolagus cuniculus). Eleven distinct Eimeria species have been identified in this host, but no PCR-based method has been developed so far for unequivocal species differentiation. In this work, we describe the development of molecular diagnostic assays that allow for the detection and discrimination of the 11 Eimeria species that infect rabbits. We determined the nucleotide sequences of the ITS1 ribosomal DNAs and designed species-specific primers for each species. We performed specificity tests of the assays using heterologous sets of primers and DNA samples, and no cross-specific bands were observed. We obtained a detection limit varying from 500fg to 1pg, which corresponds approximately to 0.8-1.7 sporulated oocysts, respectively. The test reported here showed good reproducibility and presented a consistent sensitivity with three different brands of amplification enzymes. These novel diagnostic assays will permit population surveys to be performed with high sensitivity and specificity, thus contributing to a better understanding of the epidemiology of this importan
Who reads Development of molecular assays for the identification of the 11 Eimeria species of the domestic rabbit (Oryctolagus cuniculus)?
It is typically read by researchers, students, and practitioners in Immunology and Microbiology.
- Author
- Ursula C. Oliveira; Jane S. Fraga; Dominique Licois; Michal Pakandl; Arthur Gruber
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0304-4017)
- Published
- 2011
- Language
- EN
- Field
- Immunology and Microbiology (Life Sciences)