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Can I read Fractionation of Particulate Material from Maize Coleoptile Homogenates with Polyethylene Glycol on EtoBox?
Fractionation of Particulate Material from Maize Coleoptile Homogenates with Polyethylene Glycol by Wolfgang Michalke; Brigitte Schmieder is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Fractionation of Particulate Material from Maize Coleoptile Homogenates with Polyethylene Glycol about?
Polyethyleneglycol (PEG) has been used to sediment particulate material from maize coleoptile homogenates at low centrifugal forces. The resuspended sediments were used for N-1-naphthylphthalamic acid (NPA)-binding studies. Binding activity was influenced by monovalent cations in the resuspension medium, but even at concentrations of up to 1.2 M NaCl or 0.5 M LiCl or CsCl, half of the binding activity was still recovered. Binding activity was influenced by divalent cations, because it decreased when Ca(2+) and Mg(2+) ions in the medium were complexed with EDTA. Fractionated sedimentation using increasing concentrations of PEG resulted in two peaks of NPA-binding activity at about 3% and 6% PEG. The 3% peak cintained enzymatic markers for mitochondria and endoplasmatic reticulum while the 6% peak contained NPA-binding activity only. Possible explanations for the bimodal distribution of NPA binding after fractionated PEG precipitation are discussed.
Who reads Fractionation of Particulate Material from Maize Coleoptile Homogenates with Polyethylene Glycol?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Wolfgang Michalke; Brigitte Schmieder
- Publisher
- Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0032-0935)
- Published
- 1979
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)