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Endolysin NC5 improves early cloxacillin treatment in a mouse model of Streptococcus uberis mastitis by Niels Vander Elst; Julie Bellemans; Rob Lavigne; Yves Briers; Evelyne Meyer is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract __Streptococcus uberis__ frequently causes bovine mastitis, an infectious udder disease with significant economic implications for dairy cows. Conventional antibiotics, such as cloxacillin, sometimes have limited success in eliminating __S. uberis__ as a stand-alone therapy. To address this challenge, the study objective was to investigate the VersaTile engineered endolysin NC5 as a supplemental therapy to cloxacillin in a mouse model of bovine __S. uberis__ mastitis. NC5 was previously selected based on its intracellular killing and biofilm eradicating activity. To deliver preclinical proof-of-concept of this supplemental strategy, lactating mice were intramammarily infected with a bovine __S. uberis__ field isolate and subsequently treated with cloxacillin (30.0 μg) combined with either a low (23.5 μg) or high (235.0 μg) dose of NC5. An antibiotic monotherapy group, as well as placebo treatment, was included as controls. Two types of responders were identified: fast (__n__ = 17), showing response after 4-h treatment, and slow (__n__ = 10), exhibiting no clear response at 4 h post-treatment across all groups. The high-dose combination therapy in comparison with placebo

Who reads Endolysin NC5 improves early cloxacillin treatment in a mouse model of Streptococcus uberis mastitis?

Author

Niels Vander Elst; Julie Bellemans; Rob Lavigne; Yves Briers; Evelyne Meyer

Publisher

Springer

Published

2024

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)