Can I read Glucose metabolism in freshly isolated Müller glial cells from a mammalian retina on EtoBox?

Glucose metabolism in freshly isolated Müller glial cells from a mammalian retina by Carol L. Poitry-Yamate; Marcos Tsacopoulos is a Neuroscience article available to read on EtoBox.

What is Glucose metabolism in freshly isolated Müller glial cells from a mammalian retina about?

Glucose metabolism was studied in isolated retinal Muller glial cells from the juvenile guinea pig. Cells, once enzymatically isolated and purified, were identified by morphological criteria, positive vimentin immunoreactivity, and histochemical staining for glycogen. Purified suspensions of Muller cells were obtained in quantities sufficient for biochemical analysis ( = 2 x 105/pair of retinas) and light microscopic autoradiography. In bicarbonate-buffered Ringer's medium containing 3H-2-deoxyglucose and no glucose, 2 80% of the glucose analogue taken up intracellularly by Muller cells was phosphorylated to 'H-2-deoxyglucose-6-phosphate. In autoradiographs, this non-metabolized product provided visual evidence of glucose phosphorylation: the distribution of cell grains mirrored the morphology of individual Muller cells in situ. Exposure to the glycolytic inhibitor iodoacetate (500 KM) caused an 85% decrease in adenosine triphosphate (ATP) content; concomitantly, 3H-2-deoxyglucose-6-phosphate decreased by 90% and paralleled a dramatic decrease of cell labelling in autoradiographs, while levels of 3H-2-deoxyglucose did not change. In the continual absence of glucose, glycogen conten

Who reads Glucose metabolism in freshly isolated Müller glial cells from a mammalian retina?

Author

Carol L. Poitry-Yamate; Marcos Tsacopoulos

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0021-9967)

Published

1992

Language

EN

Field

Neuroscience (Life Sciences)