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Downregulation of Cap43 gene by von Hippel-Lindau tumor suppressor protein in human renal cancer cells by Katsuaki Masuda; Mayumi Ono; Masahiro Okamoto; Wataru Morikawa; Michihiro Otsubo; Toshiro Migita; Masazumi Tsuneyoshi; Heiwa Okuda; Taro Shuin; Seiji Naito; Michihiko Kuwano is a Medicine article available to read on EtoBox.

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## Abstract We previously identified 9 genes (i.e., thymosin β4, secreted protein acidic and rich in cysteine, Cap43, ceruloplasmin, serum amyloid A, heat shock protein 90, LOT1, osteopontin and casein kinase Iγ) that are more highly expressed in cancerous regions than in noncancerous regions in human renal cancers. In our study, we considered the possibility that the von Hippel‐Lindau (VHL) tumor suppressor gene might be able to affect the expression of these 9 genes in renal cancer cells. We first established 2 VHL‐positive cell lines, 786/VHL‐1 and 786/VHL‐2, after the introduction of wild‐type VHL into VHL‐negative renal cancer 786‐O cells. Of these 9 genes, expression of the __Cap43__ gene was specifically downregulated by VHL. Expression of Cap43 was also much lower in 4 other VHL‐positive renal cancer cell lines than in VHL‐negative 786‐O cells. Cap43 promoter assays with several deletion or mutation constructs demonstrated that the Sp1 site in the element from –286 base pairs (bp) to –62 bp was partly responsible for VHL‐induced suppression of the __Cap43__ gene. Immunostaining analysis with human specimens of renal cancers demonstrated that the Cap43 protein was expressed

Who reads Downregulation of Cap43 gene by von Hippel-Lindau tumor suppressor protein in human renal cancer cells?

Author

Katsuaki Masuda; Mayumi Ono; Masahiro Okamoto; Wataru Morikawa; Michihiro Otsubo; Toshiro Migita; Masazumi Tsuneyoshi; Heiwa Okuda; Taro Shuin; Seiji Naito; Michihiko Kuwano

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

2003

Language

EN

Field

Medicine (Health Sciences)