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Lateral flow DNA biosensor for visual detection of nucleic acid with triple-helix DNA functionalized carbon nanotube 1 1 Introduction 2 2 Experimental section 2 2.1 Apparatus and reagents 2 2.2 Preparation of CNTs and CNT–P1 conjugates 3 2.3 Polyacrylamide gel electrophoresis (PAGE) to verify the generation of CNT-P1 conjugates 3 2.4 Preparation of CNT–THD conjugates 3 2.5 PAGE to verify the formation of the THD 3 2.6 Preparation of LFDB 3 2.7 Sample test steps 4 3 Results and discussion 4 3.1 Principle of THD-CNT based LFDB for tDNA detection 4 3.2 Feasibility of the proposed sensing platform 5 3.3 Parameter optimization 5 3.4 Analytical performance 6 4 Conclusion 7 CRediT authorship contribution statement 7 Declaration of competing interest 7 Data availability 7 Acknowledgements 7 Appendix A Supplementary data 7 References 7
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