Can I read Trizol-based Method for Sample Preparation and Isoelectric Focusing of Halophilic Proteins on EtoBox?
Trizol-based Method for Sample Preparation and Isoelectric Focusing of Halophilic Proteins by P.A. Kirkland; Jennifer Busby; Stan Stevens Jr.; Julie A. Maupin-Furlow is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Trizol-based Method for Sample Preparation and Isoelectric Focusing of Halophilic Proteins about?
A persisting complication in the development of well-resolved two-dimensional PAGE maps of halophilic proteins is their natural incompatibility with isoelectric focusing (IEF). The complete desalting of samples, which is necessary for IEF, tends to aggregate halophilic proteins, often requires relatively large amounts of starting material due to significant loss of sample, and is relatively time-consuming. Here, we describe a method of preparing protein samples from the haloarchaeon Haloferax volcanii that not only desalts the samples thoroughly but also drastically reduces the amount of protein loss associated with previous sample preparation methods and prevents protein aggregation during the removal of salt. This method of sample preparation, which incorporates Trizol (phenol/guanidine isothiocyanate), can easily be extended to analyze halophilic proteins from other organisms.
Who reads Trizol-based Method for Sample Preparation and Isoelectric Focusing of Halophilic Proteins?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- P.A. Kirkland; Jennifer Busby; Stan Stevens Jr.; Julie A. Maupin-Furlow
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)
- Published
- 2006
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)