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A New Approach for Pharmacokinetics of Single-dose Cetuximab in Rhesus Monkeys by Surface Plasmon Resonance Biosensor by Jinjing Che; Hui Wang; Zhihang Chen; Xin Li; Yunan Hou; Chengqi Shan; Yuanguo Cheng is a Chemistry article available to read on EtoBox.
What is A New Approach for Pharmacokinetics of Single-dose Cetuximab in Rhesus Monkeys by Surface Plasmon Resonance Biosensor about?
A novel assay method has been developed and validated, using surface plasmon resonance (SPR), for quantitation of cetuximab (C225) in monkey serum. By injecting non-labeled antibody samples onto a biosensor surface on which epidermal growth factor receptor (EGFR) was immobilized, the concentration of C225 can be accurately measured. This assay has a range of reliable response from 0.05 to 50 microg/ml C225 in monkey serum, which was well fitted with a sigmoidal model. The immobilized EGFR was found to be stable for at least 100 regeneration cycles at room temperature. Intra- and inter-assay CVs ranged from 3.20% to 8.89% and from 5.93% to 11.11%, accuracy from 92% to 107.52% and from 90% to 106.88%, respectively. Matrices such as 50% human serum, 50% Sprague Dawley rat serum, chimeric recombinant anti-CD20 monoclonal antibody, human gamma-globulin and chimeric recombinant her2 antibody did not interfere with C225 analysis on the sensor surface. This is the first report on the quantitation of C225 in monkey serum by an optical biosensor technology. This method was used to characterize the pharmacokinetics of C225 in rhesus monkeys. After a single-dose of intravenous infusion adminis
Who reads A New Approach for Pharmacokinetics of Single-dose Cetuximab in Rhesus Monkeys by Surface Plasmon Resonance Biosensor?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Jinjing Che; Hui Wang; Zhihang Chen; Xin Li; Yunan Hou; Chengqi Shan; Yuanguo Cheng
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0731-7085)
- Published
- 2009
- Language
- EN
- Field
- Chemistry (Physical Sciences)